Lexical Definition

‎Πύθων‎ Pýthōn (poo'-thone) divining spirit ^G:N-M
from (the name of the region where Delphi, the seat of the famous oracle, was located)

divination.

• a Python, i.e. (by analogy, with the supposed diviner there) inspiration (soothsaying)

Abbott-Smith

* πύθων, -ωνος, ὁ,
1. in cl., Python, a serpent slain by Apollo, who is hence surnamed the Pythian.
2. In Plut. (ii, 414 E), a name given to ventriloquist soothsayers (ἐγγαστρίμυθοι; cf. Le 19:31 20:6, 27, I Ki 28:7), and perhaps in this sense πνεῦμα πύθωνα, a python-spirit: Ac 16:16.†

Abbott-Smith References

Liddell-Scott-Jones

Πύθων [ῡ], ωνος, ὁ,

(compare Πυθώ) the serpent “Python”, slain by Apollo, [Refs 4th c.BC+]

__II παιδίσκη ἔχουσα πνεῦμα Πύθωνα a spirit of divination, [NT]
__II.2 plural Πύθωνες, ventriloquists, [Refs 1st c.AD+]

_{LSJ Scripture Refs: Acts.16.16}